Original Article
Copyright ©2011 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 7, 2011; 17(29): 3420-3430
Published online Aug 7, 2011. doi: 10.3748/wjg.v17.i29.3420
Table 1 Reverse transcription-polymerase chain reaction primer sequences
PrimerSequence (5’-3’)Product size (bp)Temperature (°C)
Notch1-ICDForward: CTAAGATCTCCTGAGGGCTTCAAAGTGTC237563
Reverse: GCGAATTCCTTGAAGGCCTCCGGAAT
H-Ras (ORF)Forward: GAGGATCCATGACGGAATATAAGCTGG66861
Reverse: GTGAATTCTCAGGAGAGCACACACTTG
Hes-1Forward: ATGAACGAGGTGACCCGCTT44362
Reverse: CTGGAAGGTGACACTGCGTT
LSF (ORF)Forward: GGAAAGATCTAGGATGGCCTGGGCTCTGAAG154759
Reverse: CAAAGCTTGGGCACGAAACGCCGCACTCCT
LSF (promoter analysis)Forward: CTAGGTACCCAACATGGTAAGATCCTGTCTCT202056
Reverse: GGAGATCTTCTCATCCCTGCTTTCTGTTTCCT
GAPDHForward: GGTGGAGGTCGGAGTCAACGGA24060
Reverse: GAGGGATCTCGCTCCTGGAGGA