α-fetoprotein involvement during glucocorticoid-induced precocious maturation in rat colon

Figure 2 Expression of α-fetoprotein protein by Western blotting analysis in colons of suckling rats treated with or without hydrocortisone as indicated in lanes, respectively. Western blotting analysis using α-fetoprotein (AFP) (C-19), revealed a 72 kDa isoform. A: AFP levels in colons of hydrocortisone-treated animals were higher than in controls of 8- and 10-d-old rats; B: Results are indicated in percentage above the β-actin value and are representative of three independent experiments. ^aP < 0.05 vs controls; C: Pups received saline; HC: Pups received hydrocortisone.