Streptozotocin-induced expression of Ngn3 and Pax4 in neonatal rat pancreatic α-cells

Figure 2 Expression and location of Ngn3. A: Immunofluorescent colocalization of Ngn3 and insulin in streptozotocin (STZ)-treated rats (d-f, j-l, p-r and v-x) and control rats (a-c, g-i, m-o and s-u) on d 8 (a-f), 12 (g-l), 16 (m-r) and 20 (s-x) after STZ treatment. Pancreatic sections were immunostained with anti-Ngn3 antibody (green) and anti-insulin antibody (red). Original magnification, × 400; B: Immunofluorescent colocalization of Ngn3 and glucagon in STZ-treated rats (d-f, j-l, p-r and v-x) and control rats (a-c, g-i, m-o and s-u) on d 8 (a-f), 12 (g-l), 16 (m-r) and 20 (s-x) after STZ treatment. Pancreatic sections were immunostained with anti-glucagon antibody (green) and anti-Ngn3 antibody (red). Original magnification, × 400; C: Proportion of Ngn3+ / glucagon+ cells in total α-cells after treatment (Y). ^aP < 0.05 vs control rats.