miRNA studies in in vitro and in vivo activated hepatic stellate cells

Figure 3 Verification of microarray data by real-time polymerase chain reaction of 11 differentially regulated miRNAs and their regulation upon in vivo activation of hepatic stellate cells. A: The graph depicts the changes in the miRNA expression of 11 miRNAs detected by real-time polymerase chain reaction, comparing quiescent with 10-d culture activated primary hepatic stellate cells (pHSCs). The data represent the mean ± SE of three independent experiments (^aP≤ 0.05, ^bP≤ 0.005); B: The graph illustrates the relative expression levels of miRNAs in isolated in vivo activated pHSCs (n = 4, choline-deficient ethionine supplemented diet) compared to normal diet (n = 2) (^aP≤ 0.05, ^bP≤ 0.005).