miRNA studies in in vitro and in vivo activated hepatic stellate cells

Figure 2 Histological and immunohistochemical analysis of livers from rats receiving choline-deficient ethionine supplemented diet for 4 wk. A: HE staining shows the structural changes between control and choline-deficient ethionine supplemented (CDE) diet livers. No severe steatosis is observed; B: The Sirius Red staining depicts the deposition of collagen around the portal area and the whole liver; C: The increase in smooth muscle α-actin (SMAA) staining reflects the increasing number of myofibroblasts seen in patches throughout the liver. Scale bar represents 200 μm; D: The Western blotting data confirm the increase in SMAA and ColI.