Enhanced proliferation, invasion, and epithelial-mesenchymal transition of nicotine-promoted gastric cancer by periostin

Figure 3 Periostin siRNA inhibited nicotine-promoted cell growth in gastric cancer cells. SGC-7901 cells with stable expression of siRNA-periostin (siRNA-P) and vacant siRNA-control (siRNA-C) were treated with nicotine. Cells were then lysed to analyze cell viability using the methylthiazolyldiphenyl-tetrazolium bromide assay at 0, 24, 48, and 72 h of treatment, and growth curves were constructed. The viability of the siRNA-C+nicotine group was significantly higher than those of the other three groups at the 48 and 72 h time points (P < 0.05), which is indicated by “a”. Notes: siRNA-C: vacant siRNA-control; siRNA-P transfected with periostin.