Switching-on of serotonergic calcium signaling in activated hepatic stellate cells

Figure 4 5-hydroxytryptamine-induced intracellular Ca²⁺ concentration transients via metabotropic 5-hydroxytryptamine₂ receptor in activated hepatic stellate cells. A, B: 5-hydroxytryptamine (5-HT)-induced intracellular Ca²⁺ concentration ([Ca²⁺]_i) transients were completely abolished by pretreatment with U73122 (1 μmol/L), a phospholipase C blocker (n = 3, 11 cells); C, D: Ritanserin (10 μmol/L), a 5-HT₂ antagonist, inhibited the [Ca²⁺]_i responses to 5-HT in activated hepatic stellate cells (2 wk-cultured cells; n = 3, 13 cells). Data are presented as the mean ± SE.