Chemometrics of differentially expressed proteins from colorectal cancer patients

Table 3 List of proteins found in 2D gel in thiourea lysis buffer extracts

Spot No.	Protein name	SwissProt No.1	MOWSE score2	MW (Da)	pI	Sequence coverage (%)	GRAVY	Average fold change3
1	Tropomyosin α-4 chain	P67936	139	28506	4.67	33	-1.033	-51.151
2	Putative tropomyosin α-3-chain-like protein	A6NL28	53	27407	4.71	25	-0.992	4.922
3	GC1q-R, mitochondrial	Q07021	123	31768	4.74	20	-0.461	-3.333
4	Calreticulin	P27797	73	47092	4.30	11	-1.191	1.394
5	Prohibitin	P35232	421	29890	5.57	41	0.024	0.032
6	Heat shock 70 kDa protein	P11021	775	72488	5.07	42	-0.487	-32.940
7	Tubulin β-2C chain	P68371	299	48142	4.70	25	-0.347	-9.060
8	PDI	P07237	266	57510	4.82	42	-0.450	-1.515
9	ATP synthase subunit β, mitochondrial	P06576	1096	56559	5.26	43	0.018	-15.661
10	ATP synthase D chain	O75947	117	18406	5.22	32	-0.569	-5.129
11	Chloride intracellular channel protein 1	O00299	299	27123	5.09	30	-0.293	20.288
12	Tubulin α-1 chain	Q71U36	61	50800	4.94	6	-0.229	-30.291
13	Apolipoprotein A-I	P02647	129	28078	5.27	37	-0.840	78.135
14	Actin, cytoplasmic 2	P63261	52	42009	5.31	4	-0.205	-26.716
15	Actin, aortic smooth muscle	P62736	261	42154	5.23	21	-0.233	46.181
16	Stomatin-like protein 2	Q9UJZ1	151	38644	6.88	28	-0.161	-29.709
17	60 kDa heat shock protein, mitochondrial	P10809	451	61386	5.70	28	-0.074	14.023
18	Triosephosphate isomerase	P60174	167	26828	6.51	24	-0.126	16.757
19	Annexin A5	P08758	195	35994	4.94	39	-0.330	-2.019
20	Cytochrome b-c1 complex subunit 1, mitochondrial	P31930	96	53342	5.94	18	-0.141	13.151
21	Annexin A3	P12429	140	36396	5.63	22	-0.430	31.244
22	Annexin A4	P09525	165	35983	5.85	33	-0.447	11.890
23	α-enolase	P06733	143	47385	6.99	12	-0.226	85.960
24	Lamin-A/C	P02545	198	65192	6.40	25	-0.947	-3.378

¹Protein accession number as SwissProt at http://www.expasy.org/uniprot;

²MOWSE score from MASCOT protein database search at http://www.matrixscience.com, where score > 41 is statistically significant (P < 0.05);

³Average ratio of the spot intensity in normal mucosa over tumor tissue (negative variation or decrease) or tumor tissue over normal tissue (positive variation or increase). PDI: Protein disulfide isomerase.