Chemometrics of differentially expressed proteins from colorectal cancer patients

Table 2 List of proteins found in 2D gel of Tris extracts

Spot No.	Protein name	Swissprot No.1	MOWSE score2	MW (Da)	pI	Sequence coverage (%)	GRAVY	Average fold change3
1	Proteasome subunit β type 6	P28072	134	25573	4.80	16	0.034	-2.967
2	14-3-3 protein ζ	P63104	336	35567	6.97	40	-0.744	11.659
3	Tropomyosin α-3C-like protein	A6NL28	127	27407	4.71	31	-0.992	44.183
4	Rho GDP-dissociation inhibitor 1	P52565	167	23120	5.03	29	-0.700	-7.607
5	14-3-3 protein ζ	P63104	282	27919	4.73	16	-0.621	4.127
6	Tubulin β-2C chain	P68371	524	50304	4.83	40	-0.362	-52.184
7	Cathepsin B	P07858	74	22981	5.20	18	-0.433	33.149
8	Rho GDP-dissociation inhibitor 2	P52566	48	22901	5.10	18	-0.799	-10.625
9	SEC13 homolog	P55735	78	36040	5.22	9	-0.372	6.873
10	Hsc70-interacting protein	P50502	164	28464	8.92	21	-0.653	20.959
11	Apolipoprotein A-I	P02647	143	30777	5.56	26	-0.717	-4.478
12	Proteasome subunit α type 3	P25788	201	15958	6.82	41	0.008	4.249
13	Actin, cytoplasmic 2	P63261	105	26169	5.65	14	-0.156	28.601
14	60 kDa heat shock protein	P10809	151	61348	5.70	14	-0.074	131.219
15	Peroxiredoxin-2	P32119	283	21935	5.67	42	-0.210	1.250
16	Guanine nucleotide binding protein subunit β 2	P62879	112	37954	5.60	11	-0.183	-14.442
17	F-actin-capping protein subunit β	P47756	259	34187	6.02	37	-0.574	33.554
18	GST-P	P09211	730	23442	5.44	60	-0.131	4.834
19	Haptoglobin-related protein	P00739	49	39529	6.42	3	-0.308	56.209
20	Cathepsin Z	Q9UBR2	100	27787	5.48	15	-0.545	-60.766
21	F-actin-capping protein subunit β	P47756	245	21280	7.93	34	-0.540	13.278
22	Actin-related protein 3	P61158	148	47704	5.61	27	-0.271	15.881
23	Abhydrolase domain-containing protein 14B	Q96IU4	200	25429	6.82	26	-0.023	0.765
24	Nucleoside diphosphate kinase A	P15531	87	19873	5.42	36	-0.075	73.120
25	L-lactate dehydrogenase B chain	P07195	228	36928	5.71	14	0.056	3.513
26	Fibrinogen β chain	P02675	151	56624	8.54	22	-0.758	41.329
27	Leukocyte elastase inhibitor	P30740	170	42857	5.90	11	-0.249	10.458
28	PDI A3	P30101	674	57202	5.98	35	-0.506	7.579
29	Gelsolin	P06396	238	86103	5.90	20	-0.415	-11.917
30	Heat shock 27 kDa protein	P04792	256	22840	5.98	47	-0.567	-1.508
31	DJ-1 protein	Q99497	122	20079	6.33	54	0.004	4.981
32	Fibrinogen β chain	P02675	75	56624	8.54	22	-0.758	-72.722
33	Selenium-binding protein 1	Q13228	502	52971	5.93	21	-0.254	-26.544
34	Selenium-binding protein 1	Q13228	592	52938	5.93	30	-0.254	27.403
35	Selenium-binding protein 1	Q13228	979	52938	5.93	37	-0.254	-1.887
36	Leukotriene A-4 hydrolase	P09960	215	69792	5.80	22	-0.259	29.759
37	Proteasome subunit α type 6	P60900	71	20988	8.57	39	-0.247	0.768

¹Protein accession number at SwissProt at http://www.expasy.org/uniprot;

²MOWSE score from MASCOT protein database search at http://www.matrixscience.com, where score > 41 is statistically significant (P < 0.05);

³Average ratio of the spot intensity in normal mucosa over tumor tissue (negative variation or decrease) or tumor tissue over normal tissue (positive variation or increase). GST-P: Glutathione S-transferase P; PDI: Protein disulfide isomerase.