Trefoil factors: Tumor progression markers and mitogens via EGFR/MAPK activation in cholangiocarcinoma

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Mar 28, 2011 (publication date) through Apr 29, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 28, 2011; 17(12): 1631-1641
Published online Mar 28, 2011. doi: 10.3748/wjg.v17.i12.1631

Table 2 Primer sequences of genomic and reverse transcription polymerase chain reaction, and polymerase chain reaction fragment sizes

Primer name	Forward (5→3)	Reverse (5→3)	Product size (bp)
Genomic-PCR
TFF1	CAGGGATCTGCCTGCATC	ATCGATCTCTTTTAATTTTTAGGCC	219
TFF2	GAAGAATCTCCCGAACCAGG	GTCACACTTCAAAAACTAGAGG	123
TFF3	CAGGCACTGTTCATCTCAGC	TATTCGTTAAGACATCAGGCTCC	129
β-actin	TCACCCACACTGTGCCCATCTACGA	CAGCGGAACCGCTCATTGCCAATGG	375
RT-PCR
TFF1	CCCGTGAAAGACAGAATT	GATCCCTGCAGAAGTGTCT	169
TFF2	CTCCTGGCAGCGCTCCTCGTC	GATGCCCGGGTAGCCACAGTTTCT	223
TFF3	AACCGGGGCTGCTGCTTTG	GAGGTGCCTCAGAAGGTGC	92
RPLP0	CTTCCCACTTGCTGAAAAG	CCAAATCCCATATCCTCGT	168

PCR: Polymerase chain reaction; RT-PCR: Reverse transcription PCR; TFF: Trefoil factor.

Citation: Kosriwong K, Menheniott TR, Giraud AS, Jearanaikoon P, Sripa B, Limpaiboon T. Trefoil factors: Tumor progression markers and mitogens via EGFR/MAPK activation in cholangiocarcinoma. World J Gastroenterol 2011; 17(12): 1631-1641
URL: https://www.wjgnet.com/1007-9327/full/v17/i12/1631.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i12.1631