Survivin isoforms and clinicopathological characteristics in colorectal adenocarcinomas using real-time qPCR

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 28, 2011; 17(12): 1614-1621
Published online Mar 28, 2011. doi: 10.3748/wjg.v17.i12.1614

Table 3 Sequences of primers and hybridization probes

	Name	Oligonucleotide sequence, 5’-3’
Forward primer	F1	CCACCGCATCTCTACATTCA
Reverse primers	WT	TATGTTCCTCTATGGGGTCG
	Sur2B	AGTGCTGGTATTACAGGCGT
	SurΔΕx3	TTTCCTTTGCATGGGGTC
Hybridization probes		CAAGTCTGGCTCGTTCTCAG TGGG-FITC¹
Hybridization probes		LCRed640-CAGTGGATGAAG CCAGCCTCG-Ph²

¹: 3’-end-labeled with fluorescein isothiocyanate (FITC);

²: 5’-end-labeled with LC Red 640; phosphorylated on the 3’ end to avoid extension of the probe.

Citation: Pavlidou A, Dalamaga M, Kroupis C, Konstantoudakis G, Belimezi M, Athanasas G, Dimas K. Survivin isoforms and clinicopathological characteristics in colorectal adenocarcinomas using real-time qPCR. World J Gastroenterol 2011; 17(12): 1614-1621
URL: https://www.wjgnet.com/1007-9327/full/v17/i12/1614.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i12.1614

Pavlidou A, Dalamaga M, Kroupis C, Konstantoudakis G, Belimezi M, Athanasas G, Dimas K. Survivin isoforms and clinicopathological characteristics in colorectal adenocarcinomas using real-time qPCR. World J Gastroenterol 2011; 17(12): 1614-1621 [PMID: 21472129 DOI: 10.3748/wjg.v17.i12.1614]

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