Epigallocatechin gallate inhibits HBV DNA synthesis in a viral replication - inducible cell line

Figure 2 Epigallocatechin gallate inhibited hepatitis B virus e antigen expression level but not hepatitis B virus surface antigen expression level in the HepG2. 117 cell line. Twenty four hours after seeding, cells were incubated with medium containing different concentrations of Epigallocatechin gallate (EGCG) for 3 d. The hepatitis B virus e antigen (HBeAg) and hepatitis B virus surface antigen (HBsAg) expression levels in the supernatants were measured using enzyme-linked immunosorbent assay kits for measuring HBeAg and HBsAg, respectively. The level of HBeAg (A) and HBsAg (B) from cells not treated with EGCG (the control) were arbitrarily designated as 1. The levels of HBeAg (A) and HBsAg (B) in EGCG-treated cells were normalized with the control and expressed as relative levels. Error bars indicate standard deviation of 3 independent experiments.