Autoantibodies against MMP-7 as a novel diagnostic biomarker in esophageal squamous cell carcinoma

Figure 2 pET-28b/matrix metalloproteinase-7 protein expression by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Panel A: Time course analysis of pET-28b/matrix metalloproteinase-7 (MMP-7) protein expression by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). M: Protein marker; 1-5: Samples for different time points (1, 2, 4, 6, and 7 h) at 37°C after 0.4 mmol/L IPTG induced; 6: Uninduced bacterial lysate. Panel B: SDS-PAGE showing pET-28b/MMP-7 protein expression induced by different amounts of IPTG. 1, 5: Uninduced bacterial lysate; 2-4: Samples induced at different concentrations of IPTG (0.4, 0.8, 1 mmol/L) at 37°C for 4 h; 6-8: Samples induced at different concentrations of IPTG (0.4, 0.8, 1 mmol/L) at 28°C for 4 h.