Involvement of PI3K and ERK1/2 pathways in hepatocyte growth factor-induced cholangiocarcinoma cell invasion

Figure 6 Suppression of HGF-induced cholangiocarcinoma cell invasiveness by PI3-kinase inhibitor, LY294002. HuCCA-1 and KKU-M213 cells were treated with 50 ng/mL HGF in the absence (control) or presence of 10 and 50 μmol/L LY294002 for 6 h, and subsequently Akt phosphorylation was determined by Western blotting (A). In vitro invasion of HuCCA-1 (B) and KKU-M213 (C) cells was evaluated in the absence or presence of HGF with or without 50 μmol/L LY294002. Numbers of invaded cells are presented as mean ± SE of results obtained from three independent experiments. ^bP < 0.01 vs control.