Differential changes in intrinsic innervation and interstitial cells of Cajal in small bowel atresia in newborns

Figure 1 Immunohistochemistry of a full-thickness section of the ileum. A: Protein gene product (PGP) 9.5 in the ileum proximal to the atresia shows a dense innervation within the submucosal and myenteric plexus; B: PGP 9.5 in the ileum distal to the atresia shows a normal innervation pattern of the submucosal and myenteric plexuses; C: S-100 in the ileum proximal to the atresia shows numerous fibers and large ganglia (> 20 μm) within the submucosal plexus and normal innervation within the myenteric plexus; D: S-100 in the ileum distal to the atresia shows a normal innervation pattern within the submucosal and myenteric plexuses; E: c-kit in the ileum proximal to the atresia shows that myenteric and muscular interstitial cells of Cajal (ICCs) are almost absent; F: c-kit in the ileum distal to the atresia shows normal distribution of myenteric and muscular ICCs.