Differential changes in intrinsic innervation and interstitial cells of Cajal in small bowel atresia in newborns

Table 2 Distribution and density of immunostaining (proximal vs distal)

	Proximal				Distal
	SP	CM	MP	LM	SP	CM	MP	LM
PGP 9.5	++	+	++	+	++	+	++	+
S-100	+++	+	+++	+	++	+	++	+
NF	++	+	++	+	++	+	++	+
c-Kit (ICCs)	-	-/+	+/-	-	-	+	++	+

PGP: Protein gene product; ICCs: Interstitial cells of Cajal; SP: Submucosal plexus; CM: Circular muscle; MP: Myenteric plexus; LM: Longitudinal muscle; NF: Neurofilament; -: No expression (no staining); +: Low expression (few neuronal fibers and cells/few ICCs); ++: Moderate expression (numerous neuronal fibers and cells/numerous ICCs); +++: High expression (dense networks of neuronal fibers and cells/dense ICCs networks).