Smad7 dependent expression signature highlights BMP2 and HK2 signaling in HSC transdifferentiation

Table 3 Comparison of gene regulation in activated hepatic stellate cells in vivo (De Minicis et al[13])

Gene symbol	Smad7 overexpressing HSCs	In vivo activated untransformed HSCs
Acta2	↓	↑
BMP21	↑	↓
Cnn1	↓	↑
Col1a1	↓	↑
Col3a1	↑	↑
Cryab	↓	↓
Cyp1b1	↓	↑
Ddah1	↓	↑
Ednrb	↑	↑2
Eno2	↓	↓
Ephx1	↑	↑2
Fn1	↓	↑
Gabbr1	↓	↓
Hgf	↑	↑2
Hk21	↓	↑
Hsd11b1	↑	↑2
Id1	↓	↓
Igfbp3	↑	↑2
Kif4	↑	↑
Lox	↓	↑
Lpl	↓	↑2
Lum	↓	↑
Ncam1	↓	↑
P4ha1	↓	↑
Pdgfra	↑	↓
Pfkp	↓	↑
Plod2	↓	↑
Rasl11b	↓	↑
Serping1	↑	↑
Slc16a3	↓	↑
Slc2a1	↓	↑
Sod3	↑	↑
Tmepai_predicted	↓	↓2
Tnc	↓	↓2
Tnnt2	↓	↑
VEGFa1	↓	↑ (VEGFc)1
Wfdc1	↓	↑2

Gene expression profiles during hepatic stellate cell activation in culture and in vivo (Gastroenterology 2007; 132: 1937-1946[13]) and Smad7 overexpressing hepatic stellate cells (HSCs) (our study).

¹Genes regulated the strongest in our study are marked;

²The regulation of in vivo activated HSCs which is different compared to culture activated HSCs in De Minicis study. In total 37 genes overlap in both studies, 22 of those (60%) are oppositely regulated indicating participation of transforming growth factor-β/Smad7 on the regulation of those genes in vivo.