HNF-4α determines hepatic differentiation of human mesenchymal stem cells from bone marrow

Figure 2 Detection of liver-specific genes and liver-enriched transcription factors in induced mesenchymal stem cells by reverse transcription-polymerase chain reaction. A: Induced mesenchymal stem cells at the indicated time point: 0, 1, 4, 7 and 10 wk post-induction; B: Human hepatoma cell lines: 1, HepG2; 2, HA22T/VGH; 3, SK-Hep-1; and 4, negative control. Expression of β2M was used as the control for adjustment of sample amount. Alb: Albumin; AFP: α-fetoprotein; TAT: Tyrosine-aminotransferase; TO: Tryptophan 2,3-dioxygenase; G6P: Glucose 6-phosphatase; β2M: β-2-microglobulin.