HNF-4α determines hepatic differentiation of human mesenchymal stem cells from bone marrow

Table 1 Primer sets used for reverse transcription-polymerase chain reaction analysis

Gene	Forward primer	Reverse primer	Product size (bp)
Alb	TGCTTGAATGTGCTGATGACAGGG	AAGGCAAGTCAGGGCATCTCATC	161
AFP	TGCAGCCAAAGTGAAGAGGGAAGA	CATAGCGAGCAGCCCAAAGAAGAA	216
TAT	TGAGCAGTCTGTCCACTGCCT	ATGTGAATGAGGAGGATCTGAG	358
G6P	GCTGGAGTCCTGTCAGGCATTGC	TAGAGCTGAGGCGGAATGGGAG	350
TO	ATACAGAGACTTCAGGGAGC	TGGTTGGGTTCATCTTCGGTATC	299
HNF-1α	GTGTCTACAACTGGTTTGCC	TGTAGACACTGTCACTAAGG	251
HNF-3β	CACCACTACGCCTTCAACC	GGTAGTAGGAGGTATCTGCGG	235
HNF-4	CTGCTCGGAGCCACAAAGAGATCCATG	ATCATCTGCCACGTGATGCTCTGCA	371
C/EBPα	CAAGAAGTCGGTGGACAAGAAC	CCTCATCTTAGACGCACCAAGT	450
β2M	TGACGTGTGAACCATG	TGGAGACAGCACTCAAAG	242

The thermo-cycling parameters for β2M: 94°C for 5 min/28 cycles of 94°C for 30 s, 56°C for 30 s, and 72°C for 30 s/72°C for 10 min; The thermo-cycling parameters for other genes: 94°C for 5 min/38 cycles of 94°C for 30 s, 56-58°C for 30 s, and 72°C for 30 s/72°C for 10 min. Alb: Albumin; AFP: α-fetoprotein; TAT: Tyrosine-aminotransferase; TO: Tryptophan 2,3-dioxygenase; G6P: Glucose 6-phosphatase; β2M: β-2-microglobulin..