High expression of ErbB2 contributes to cholangiocarcinoma cell invasion and proliferation through AKT/p70S6K

Figure 2 Invasion motility and proliferation of three cholangiocarcinoma cell lines. A: Cells resuspended in 0.2% fetal bovine serum (FBS) medium were plated in the upper compartment of a Transwell chamber coated with and without Matrigel, for invasion and motility assays. The lower compartment was filled with 10% FBS medium. After 6 h incubation, numbers of invading/motile cells were counted; B: Cells were plated on 96-well plates in 10% FBS medium. After incubation for 12-72 h, cell survival was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Data are presented as mean ± SE of the percentage relative to controls obtained from three separate experiments.