Helicobacter pylori HopE and HopV porins present scarce expression among clinical isolates

Figure 4 Frequency of recognition of 4 H. pylori antigens by human sera. Bars represent number of immunoreactive human sera from a panel of 69 H. pylori-infected patients showing IgG- and IgA-type immunoreactivity tested by Western blotting assays on E. coli lysates expressing separately HopE, HopV, CagA and VacA cloned antigens. A: Frequency of IgA- and IgG-type immune responses (as number of sera) which reacted with lysates containing one of these antigens; B: IgA response data taken from panel A, with patients separated by age into 2 groups: under 18 years old and adults; C: IgG response data taken from panel A, separated by age as above. As negative controls, sera from 8 non-infected patients did not display any immune response when tested with these antigens (not shown). Fisher’s test was used for statistical analysis, and significance lower than 0.05 is indicated.