Aberrant gene methylation in the peritoneal fluid is a risk factor predicting peritoneal recurrence in gastric cancer

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jan 21, 2010; 16(3): 330-338
Published online Jan 21, 2010. doi: 10.3748/wjg.v16.i3.330

Table 1 Primer and probe sequence of the LightCycler system for q-MSP

Gene		Primer sequence	Ref.
CHFR	Forward	TTTCGTGATTCGTAGGCGAC	[33]
	Reverse	CGACAACTAAAACGAAACCGA
	Probe	5' FAM-CGCGAAAATAAACGCGTAAAAAACGCTCG-3'BHQ
p16	Forward	TGGAATTTTCGGTTGATTGGTT	[34]
	Reverse	AACAACGTCCGCACCTCCT
	Probe	5' FAM- ACCCGACCCCGAACCGCG -3'BHQ
RUNX3	Forward	CGTTCGATGGTGGACGTGT	[35]
	Reverse	GACGAACAACGTCTTATTACAACGC
	Probe	5' FAM-CGCACGAACTCGCCTACGTAATCCG-3'BHQ
E-cadherin	Forward	AATTTTAGGTTAGAGGGTTATCGCGT	[34,36]
	Reverse	TAACTAAAAATTCACCTACCGAC
	Probe	5' FAM-CGCCCACCCGACCTCGCAT-3'BHQ
hMLH1	Forward	CGTTATATATCGTTCGTAGTATTCGTGTTT	[34]
	Reverse	CTATCGCCGCCTCATCGT
	Probe	5' FAM-CGCGACGTCAAACGCCACTACG-3'BHQ
ABCG2	Forward	TTGGGTAATTTGTGCGTTA
	Reverse	CTACGAAAATCACCAAACGCTC
	Probe	5' FAM-TTAATCGCCGTCACTAACCGA-3'BHQ
BNIP3	Forward	TAGGATTCGTTTCGCGTACG	[37]
	Reverse	ACCGCGTCGCCCATTAACCGCG
	Probe	5' FAM-CGTAAAATACGTATAACACGTACGAC-3'BHQ
β-actin	Forward	TGGTGATGGAGGAGGTTTAGTAAGT	[34]
	Reverse	AACCAATAAAACCTACTCCTCCCTTAA
	Probe	5' FAM-ACCACCACCCAACACACAATAACAAACACA-3'BHQ

Citation: Hiraki M, Kitajima Y, Sato S, Nakamura J, Hashiguchi K, Noshiro H, Miyazaki K. Aberrant gene methylation in the peritoneal fluid is a risk factor predicting peritoneal recurrence in gastric cancer. World J Gastroenterol 2010; 16(3): 330-338
URL: https://www.wjgnet.com/1007-9327/full/v16/i3/330.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i3.330

Hiraki M, Kitajima Y, Sato S, Nakamura J, Hashiguchi K, Noshiro H, Miyazaki K. Aberrant gene methylation in the peritoneal fluid is a risk factor predicting peritoneal recurrence in gastric cancer. World J Gastroenterol 2010; 16(3): 330-338 [PMID: 20082478 DOI: 10.3748/wjg.v16.i3.330]

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