Aberrant gene methylation in the peritoneal fluid is a risk factor predicting peritoneal recurrence in gastric cancer

Table 1 Primer and probe sequence of the LightCycler system for q-MSP

Gene		Primer sequence	Ref.
CHFR	Forward	TTTCGTGATTCGTAGGCGAC	[33]
	Reverse	CGACAACTAAAACGAAACCGA
	Probe	5' FAM-CGCGAAAATAAACGCGTAAAAAACGCTCG-3'BHQ
p16	Forward	TGGAATTTTCGGTTGATTGGTT	[34]
	Reverse	AACAACGTCCGCACCTCCT
	Probe	5' FAM- ACCCGACCCCGAACCGCG -3'BHQ
RUNX3	Forward	CGTTCGATGGTGGACGTGT	[35]
	Reverse	GACGAACAACGTCTTATTACAACGC
	Probe	5' FAM-CGCACGAACTCGCCTACGTAATCCG-3'BHQ
E-cadherin	Forward	AATTTTAGGTTAGAGGGTTATCGCGT	[34,36]
	Reverse	TAACTAAAAATTCACCTACCGAC
	Probe	5' FAM-CGCCCACCCGACCTCGCAT-3'BHQ
hMLH1	Forward	CGTTATATATCGTTCGTAGTATTCGTGTTT	[34]
	Reverse	CTATCGCCGCCTCATCGT
	Probe	5' FAM-CGCGACGTCAAACGCCACTACG-3'BHQ
ABCG2	Forward	TTGGGTAATTTGTGCGTTA
	Reverse	CTACGAAAATCACCAAACGCTC
	Probe	5' FAM-TTAATCGCCGTCACTAACCGA-3'BHQ
BNIP3	Forward	TAGGATTCGTTTCGCGTACG	[37]
	Reverse	ACCGCGTCGCCCATTAACCGCG
	Probe	5' FAM-CGTAAAATACGTATAACACGTACGAC-3'BHQ
β-actin	Forward	TGGTGATGGAGGAGGTTTAGTAAGT	[34]
	Reverse	AACCAATAAAACCTACTCCTCCCTTAA
	Probe	5' FAM-ACCACCACCCAACACACAATAACAAACACA-3'BHQ