Hypoxia inducible factor-1α accumulation in steatotic liver preservation: Role of nitric oxide

Figure 3 Endothelial nitric oxide synthase immunohistochemistry in steatotic and non-steatotic livers. Control livers and livers stored in University Wisconsin (UW) solution show only a little positivity for endothelial nitric oxide synthase (eNOS), localized typically in a few sinusoidal endothelial cells (arrows). The positivity increases when the livers are submitted to cold storage with IGL-1 solution and even more when trimetazidine (TMZ) is added to IGL-1 solution. When TMZ is added to IGL solution, some positive Kupffer cells (arrowhead) can be observed also. The addition of L-NAME to IGL-1 + TMZ solution reduced eNOS activity.