Hypoxia inducible factor-1α accumulation in steatotic liver preservation: Role of nitric oxide

Figure 1 Hypoxia-inducible factor-1α protein levels in liver after 24 h of cold storage. A: Representative Western (top) and densitometric analysis (bottom); B: Hypoxia-induced factor-1α (HIF-1α) protein levels quantified by Trans AM HIF-1α kit; Alanine transaminase (ALT) (C) and aspartate transaminase (AST) (D) levels in flushing effluent after 24 h of cold storage. University Wisconsin (UW): Liver preserved in UW solution; IGL-1: Liver preserved in IGL-1 solution; IGL-1 + trimetazidine (TMZ) (trimetazidine): Liver preserved in IGL-1 solution with TMZ; Cont 1: Liver flushed without cold preservation. ^aP < 0.05 vs Cont 1; ^cP < 0.05 vs UW; ^eP < 0.05 vs IGL-1.