Galangin induces apoptosis of hepatocellular carcinoma cells via the mitochondrial pathway

Figure 4 Effects of galangin-treated hepatocellular carcinoma cells on protein expression involved in the mitochondria pathway. A: The changes of Bax in the mitochondria; B: The changes of cytochrome c and apoptosis-inducing factor (AIF) in the cytosol; C: Caspase-3 and caspase-9 activation, and Poly(ADP-ribose) polymerase (PARP) cleavage induced by galangin. 1: Blank control group; 2: Dimethyl sulfoxide-treatment groups; 3: Cells were treated with 46.25 μmol/L galangin for 24 h; 4: Cells were treated with 92.5 μmol/L galangin for 24 h; 5: Cells were treated with 185 μmol/L galangin for 24 h; 6: Cells were treated with 370 μmol/L galangin for 24 h.