Apoptotic activity of caged xanthones from Garcinia hanburyi in cholangiocarcinoma cell lines

Figure 4 The four caged xanthones induce up-regulation of Bax with down-regulation of Bcl-2 and survivin gene expressions in CCA cell lines. KKU-100 and KKU-M156 cells were treated with DMSO or the four caged xanthones for the indicated times. Real-time polymerase chain reaction for each gene was performed as described in Materials and Methods. Data were expressed as mean ± SE fold increase/decrease in mRNA expression compared to control, normalized with GAPDH (n = 3). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control. All four compounds showed the same result. A: The histograms of the isomorellinol-treated cells were used as a representative; B: Bax/Bcl-2 ratio; C: Relative survivin gene expression compared to control.