Molecular determinants of the antitumor effects of trichostatin A in pancreatic cancer cells

Figure 5 Effects of TSA on BAX and p21^Waf1 expression in PC cell lines. A, B: The cell lines were incubated with TSA for 8 h as indicated. The mRNA expression of Bax (A), p21^Waf1 (B) and the housekeeping gene GAPDH (A and B) was analyzed by real time PCR, and relative amounts of target mRNA were determined. 100% mRNA expression of each gene corresponds to cells cultured without TSA. Data of ≥ 6 independent experiments were used to calculate mean values and SE; ^aP < 0.05 vs control cultures, ^cP < 0.05 vs BxPC-3 cells (Mann-Whitney test); C: BxPC-3, AsPC-1 and CAPAN-1 cells were treated with TSA at concentrations up to 10 × 10^-7 mol/L for 24 h. Expression of the p21^Waf1 protein and actin (housekeeping control protein) was analyzed by immunoblotting. Fluorescence signal intensities of p21^Waf1 and actin were quantified, and the ratio p21^Waf1/actin was determined. A ratio of 100% corresponds to control cells cultured without TSA. Data of 8 independent experiments were used to calculate mean values and SE; ^aP < 0.05 vs control cultures, ^cP < 0.05 vs BxPC-3 cells (Wilcoxon’s rank sum test).