Original Article
Copyright ©2010 Baishideng.
World J Gastroenterol. Jan 7, 2010; 16(1): 30-41
Published online Jan 7, 2010. doi: 10.3748/wjg.v16.i1.30
Table 1 Differentially expressed proteins before and after antibiotic/probiotic administration
Spot IDSwiss-ProtAcc. No.Protein nameCOG1Subcellular locationTheoretical Mr/pIExperimentalMr/pIMethod of identification2Change in protein expression with AB/PB treatment3
Pouchitis/antibiotic-induced remission
1P27797Calreticulin (CALR)OEndoplasmic reticulum48.14/4.2968.52/4.35GM (Swiss-2D PAGE)Up
2P1102178 kDa glucose-regulated protein (GRP78)OEndoplasmic reticulum72.33/5.0773.88/4.95GM (Swiss-2D PAGE)Down
3P1080960 kDa heat shock protein, mitochondrial precursor (HSP60)OMitochondrial matrix61.05/5.7060.20/5.32GM (Swiss-2D PAGE)Up
4P11142Heat shock cognate 71 kDa protein (HSPA8)ONucleolus70.90/5.3769.20/5.18GM[20]Up
5P38646Stress-70 protein, mitochondrial precursor (75 kDa glucose-regulated protein) (GRP75)OMitochondrion73.68/5.8771.41/5.70GM (Swiss-2D PAGE)Down
6Q9BU08Putative uncharacterized protein, fragment (CCT5)SUndefined59.47/5.4560.46/5.58GM[21]Up
7P02787Serotransferrin precursor (TF)PExtracellular77.05/6.8179.49/7.09GM (Swiss-2D PAGE)Down
8Q16822Phosphoenolpyruvate carboxykinase (GTP), mitochondrial precursor (PCK2)CMitochondrion70.73/7.5671.67/7.62GM[19]Up
9P68371Tubulin β-2C chain (TUBB)ZCytoplasm49.83/4.7952.44/4.79MALDI-TOF MSUp
10P06576ATP synthase subunit β, mitochondrial precursor (ATP5B)CMitochondrion56.56/5.2648.675.01MALDI-TOF MSUp
11Q8NBS9Thioredoxin domain-containing protein 5, precursor (TXNDC5)REndoplasmic reticulum47.63/5.6349.43/5.09GM[20]Down
12P35900Keratin, type I cytoskeletal 20 (KRT20)WCytoplasm48.49/5.5248.15/5.54GM[19]Down
13P06733α-enolase (ENO1)GCytoplasm47.17/7.0146.80/7.57MALDI-TOF MSDown
14P11177Pyruvate dehydrogenase E1 component subunit β, mitochondrial precursor (PDHB)CMitochondrion39.25/6.2032.96/5.64MALDI-TOF MSDown
15P17707S-adenosylmethionine decarboxylase proenzyme (AMD1)TCytoplasm38.34/5.7131.91/5.74MALDI-TOF MSUp
16P13804Electron transfer flavoprotein subunit α, mitochondrial precursor (ETFA)CMitochondrion35.08/8.6234.01/7.91GM[20]Up
17P21796Voltage-dependent anion-selective channel protein 1 (VDAC1)PMitochondrion30.77/8.6230.60/9.20GM[19]Down
18P07339Cathepsin D, precursor (CTSD)OLysosome44.55/6.1028.02/5.70GM (Swiss-2D PAGE)Down
19Q99439Calponin-2 (CNN2)ZCytoplasm33.70/6.9429.64/7.55MALDI-TOF MSUp
20P00915Carbonic anhydrase I (CA1)RCytoplasm28.87/6.5927.52/7.45GM[19]Down
21P62937Peptidyl-prolyl cis-trans isomerase A (PPIA)OCytoplasm18.01/7.6816.42/8.09GM[19]Up
22P12104Fatty acid-binding protein, intestinal (FABP2)ICytoplasm15.21/6.6214.07/6.99MALDI-TOF MSUp
23P51161Ileal lipid binding protein (FABP6)ICytoplasm14.37/6.2913.58/7.22MALDI-TOF MSUp
24P09382Galectin-1 (LGALS1)WExtracellular14.72/5.3313.25/5.26MALDI-TOF MSDown
25P07148Fatty acid-binding protein, liver (FABP1)ICytoplasm14.21/6.6012.13/6.80MALDI-TOF MSUp
26Q5T1C5Protein S100-A10 (S100A10)RPlasma membrane11.20/6.8210.52/7.25MALDI-TOF MSUp
Non-inflamed pouch/probiotic-treated pouch
1P18206Vinculin (VCL)ZCytoplasm123.80/5.50114.44/5.81GM (Swiss-2D PAGE)Up
2P27797CALROEndoplasmic reticulum48.14/4.2968.52/4.35GM (Swiss-2D PAGE)Down
3P28331NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor (NDUFS1)CMitochondrion79.47/5.8977.54/5.52GM[21]Down
4P11142HSPA8ONucleolus70.90/5.3769.20/5.18GM[20]Up
5P38646GRP75OMitochondrion73.68/5.8771.41/5.70GM (Swiss-2D PAGE)Down
6P02787TFPExtracellular77.05/6.8179.49/7.09GM (Swiss-2D PAGE)Down
7Q16822PCK2CMitochondrion70.73/7.5671.67/7.62GM[19]Up
8Q71U36Tubulin α-1A chain (TUBA1A)ZCytoplasm50.15/4.9456.47/4.82GM[17]Down
9Q8NBS9TXNDC5REndoplasmic reticulum47.63/5.6349.43/5.09GM[20]Down
10P35900KRT20WCytoplasm48.49/5.5248.15/5.54GM[19]Down
11P06733ENO1GCytoplasm47.17/7.0146.80/7.57MALDI-TOF MSDown
12P12532Creatine kinase, ubiquitous mitochondrial precursor (CKMT1B)CMitochondrion47.04/8.6043.16/8.48GM[20]Down
13P22695Cytochrome b-c1 complex subunit 2, mitochondrial precursor (UQCRC2)CMitochondrion48.44/8.7444.10/8.83GM[21]Up
14P11177PDHBCMitochondrion39.25/6.2032.96/5.64MALDI-TOF MSUp
15P17707AMD1TCytoplasm38.34/5.7131.91/5.74MALDI-TOF MSUp
16P00918Carbonic anhydrase II (CA2)RCytoplasm29.25/6.8730.75/7.69MALDI-TOF MSDown
17P60174Triosephosphate isomerase (TPI1)GCytoplasm26.67/6.4526.14/7.32MALDI-TOF MSUp