Role of the receptor for advanced glycation end products in hepatic fibrosis

Table 3 Expression of RAGE and ECM-related genes after incubation of HSC with AGE

	BSA	AGE-BSA	CML-BSA
RAGE	1.00 ± 0.14	1.20 ± 0.11	1.26 ± 0.07
TGF-β1	1.00 ± 0.06	1.12 ± 0.21	0.99 ± 0.07
Procollagen-α1(I)	1.00 ± 0.08	1.23 ± 0.06a	1.12 ± 0.19
α-SMA	1.00 ± 0.14	1.20 ± 0.20	1.16 ± 0.04
MMP-13	1.00 ± 0.05	1.09 ± 0.14	0.97 ± 0.09

CFSC-2G HSC were incubated with 50 μg/mL BSA, AGE-BSA, or CML-BSA for 24 h. RAGE, TGF-β1, procollagen-α1(I), α-SMA, and MMP-13 transcript levels are expressed relative to GAPDH and as n-fold increase compared to cells treated with BSA alone (means ± SD of at least three individual experiments).

^aP < 0.05 vs BSA. ECM: Extracellular matrix; HSC: Hepatic stellate cells; AGE: Advanced glycation end product; BSA: Bovine serum albumin; CML: N^ε-(carboxymethyl) lysine; TGF: Transforming growth factor.