Expression of γ-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116

Figure 2 The siRNA plasmid can specifically knock down γ-synuclein expression in the HCT116 cells. A: γ-synuclein mRNA expression detected by Q-RT-PCR after transient transfection. Values were γ-synuclein/GAPDH expression of one group cells relative to that of parental HCT116 cells; B: The vision of HCT116 stable transfectants in fluorescence microscopy and light microscopy; C: γ-synuclein protein expression detected by western blotting after selection of stable transfectants. Control: Parental HCT116 cells; pGCsi-vector: HCT116/vector cells; siRNA1 (2,3): HCT116/siRNA1 (2,3) cells.