Characterization of CD133+ parenchymal cells in the liver: Histology and culture

Figure 9 Relationship between CD133⁺ cells and SP phenotype. A: The percentages of CD133⁺ cells in SP and non-SP fractions of HuH7 cells were examined using Hoechst 33342 and a PE-conjugated antibody to CD133. The ratio of CD133⁺ cells was almost the same in the SP and non-SP fractions; B: HuH7 cells were sorted into four populations: SP/CD133⁺, SP/CD133^-, non-SP/CD133⁺, and non-SP/CD133^-. After 4 wk subculture, CD133⁺ and CD133^- cells were generated at similar levels from all populations.