Iron increases HMOX1 and decreases hepatitis C viral expression in HCV-expressing cells

Figure 5 Effects of FeNTA on intracellular ROS in Huh-7, 9-13, and CNS3 cells. A: Fluorescence intensity with the H₂DCF-DA probe in Huh-7 cells; B: Fluorescence intensity with the H₂DCF-DA probe in 9-13 cells; C: Fluorescence intensity with the H₂DCF-DA probe in CNS3 cells; D: Fluorescence intensity with the (control) DCF-DA in CNS3 cells. Cells were preincubated with 100 μmol/L H₂DCF-DA or DCF-DA for 30 min, and then exposed to selected concentrations of FeNTA (0, 50, 100 μmol/L) for 1 h. Intracellular ROS production was measured as described in Materials and Methods. Data represent fluorescence intensity measured and expressed as relative fluorescence units per milligram total protein (mean ± SE, n = 3 experiments). ^aP < 0.05 vs control.