Specific activation of 2'-5'oligoadenylate synthetase gene promoter by hepatitis C virus-core protein: A potential for developing hepatitis C virus targeting gene therapy

Figure 1 Expression of HCV-core in the stable cell line L02/core. A: Detection of HCV-core mRNA by RT-PCR. 572 bp visible fragments consistent with the predicted size of HCV-core mRNA were detected in L02/core cell clones. 1: DL2000 marker; 2, 3: L02 cells transfected with pcDNA3.1-core (L02/core); 4: L02 cells transfected with pcDNA3.1 (empty vector); 5: Untransfected L02 cells; B: Detection of HCV-core protein by Western blotting. 1, 2: L02/core cells; 3: L02 cells transfected with empty vector; 4: Untransfected L02 cells.