Rapid detection of intestinal pathogens in fecal samples by an improved reverse dot blot method

Table 3 Oligonucleotide probes used in the present study

Probe	Sequence (5’→3’)	Target
1	GGGAGTAAAGTTAATACCTTTGCTCATTGA	Salmonella spp.
2	CACACTGGAACTGAGACACGGTCGAGACTCCTACG GGA	Bacterial universal probe
3	CGTACCATTTGCTACGGAATAACTCAGGGAAACTTGTG	Brucella spp.
4	TCACCCCATAAAAGAGGCTCCCACTGC	E. coli O157:H7
5	TATAAGAGAATCGCATGATTTTCTTATCCAAAGATTTAT	C. botulinum
6	TGCTAGTTGAATAAGCTGGCACCTTGACG	B. cereus
7	ATGGCATCATCATTCAACCATTGGAGCAATCCGCTATGAGATGGACCC	C. perfringens
8	GGTTTCAGGTTCTTTTTCACTCCCCTCGCCG	Common probe for Shigella and Salmonella spp.
9	AAACGAGTTATCTGAACCTTCGGGGAACGATAACGG	V. parahaemolyticus
10	GAAGGCCTTTTCGATAATGATACCGGCGCTCTGCTCTCCC	Shigella spp. and Enteroinvasive E. coli
11	GGTGTTGTGGTTAATAACCGCAGCAATTGA	Shigella spp.
12	CTTCAATAATGCCAGCAGCTCCAACCCCGAAATAGATA	Salmonella typhi
13	CATAAAGGTTAATAACCTTTGTGATTGACGT	Y. enterocolitica
14	GCGGCAGCGGGAAGTAGTTTACTACTTTGCCGG	Yersinia spp.
15	CAGCACAGAGGAACTTGTTCCTTGGGTGGCGAG	V. cholerae
16	TGTTGTTAGAGAAGAACAAGGATAAGAGTAACTGCT	L. monocytogenes
17	ACATATGTGTAAGTAACTGTGCACATCTTGACGGTA	S. aureus
P	TTTGGCTAACTCCGTGCCAGCAGCCGCG	Positive control
C	BIOTIN-CCGCTGTATCACAAGGGCTGGTACCTTT	Color control
N	TTTCCGCTGTATCACAAGGGCTGGTACC	Negtive control
B	0.5 mol/L sodium bicarbonate buffer	Blank control