Involvement of 90-kuD ribosomal S6 kinase in collagen type I expression in rat hepatic fibrosis

Figure 7 Effect of p90RSK siRNA on the proliferation of HSCs. A: p90RSK siRNA inhibited the proliferation of HSC-T6 cells. Cell growth curves of the recombinant cells with or without p90RSK siRNA were analyzed by MTS conversion; B: p90RSK siRNA abolished the effect of PDGF-BB on the proliferation of HSCs. rhPDGF-BB was added to the medium at a final concentration of 20 &mgr;g/L in HSC-T6 cells transfected with pAVU6-siRSK (RNAi), which showed decreased proliferative activity compared to sham transfected control cells (control). Each sample was tested in triplicate and error bars were included.