Bile-acid-activated farnesoid X receptor regulates hydrogen sulfide production and hepatic microcirculation

Figure 4 CSE expression/activity is regulated with an FXR ligand in vivo. A: FXR +/+ and FXR -/- mice were treated for 3 d with vehicle or with 6E-CDCA 5 mg/kg body weight. Total RNA from liver of FXR +/+ and FXR -/- mice was subjected to real-time PCR quantification of CSE gene expression. ^aP < 0.05 versus FXR +/+ control mice; B: FXR +/+ and FXR -/- mice were treated for three days with vehicle or with 6E-CDCA 5 mg/kg body weight. Livers from FXR +/+ and FXR -/- mice were homogenized in cold PBS to evaluate CSE activity. ^aP < 0.05 versus FXR +/+ control mice. ^cP < 0.05 versus FXR -/- control mice; C: FXR +/+ and FXR -/- mice were treated for 3 d with vehicle or with 6E-CDCA 5 mg/kg body weight. Livers from FXR +/+ and FXR -/- mice were homogenized in cold PBS to evaluate H₂S production. ^aP < 0.05 versus FXR +/+ control mice. Data represent the mean ± SD of six experiments.