Correlation of p53 over-expression and alteration in p53 gene detected by polymerase chain reaction-single strand conformation polymorphism in adenocarcinoma of gastric cancer patients from India

Table 1 Detail of the primers and cycling parameters

p53 exons	Sequence (5’-3’)	Cycling parameters1	PCR product (bp)
5	Sp5F: TGTTCACTTGTGCCCTGACT	45 s denaturation at 94°C, 30 s annealing at 54°C, 1 min extension at 72°C	266
	Sp5R: CAGCCCTGTCGTCTCTCCAG
6	Sp6F: GCCTCTGATTCCTCACTGAT	45 s denaturation at 94°C, 30 s annealing at 53°C, 1 min extension at 72°C	160
	Sp6R: TTAACCCCTCCTCCCAGAGA
7	Sp7F: ACTGGCCTCATCTTGGGCCT	45 s denaturation at 94°C, 30 s annealing at 56°C, 1 min extension at 72°C	180
	Sp7R: TGTGCAGGGTGGCAAGTGGC
8	Sp8F: TAAATGGGACAGGTAGGACC	45 s denaturation at 94°C, 30 s annealing at 54°C, 1 min extension at 72°C	230
	Sp8R: TCCACCGCTTCTTGTCCTGC

¹Initial denaturation of 10 min at 94°C and final extension of 10 min at 72°C were used in all amplifications. Number of cycles in all amplifications was 35.