Synthesis of platelet-activating factor and its receptor expression in Kupffer cells in rat carbon tetrachloride-induced cirrhosis

Figure 4 A: Saturation curve of ¹²⁵I-ET-1 binding to cirrhotic and normal rat Kupffer cells. ¹²⁵I-ET-1 at a concentration between 6.25-800 pmol/L was incubated with Kupffer cells (1 × 10⁶), in presence or absence of 2 &mgr;mol/L unlabeled ET-1 at 20°C for 3 h; B: Scattered blot analysis of ¹²⁵I-ET-1 binding to cirrhotic and normal rat Kupffer cells. The result was analyzed by scatchard plot. Cirrhosis: R = 0.98, Kd = 37.72 pmol/L, Bax = 1639 ± 67.7 fmol/mg. DNA; Control: R = 0.94, Kd = 22.79 pmol/L, Bax = 820.4 ± 28.7 fmol/mg. DNA.