Influence of quasispecies on virological responses and disease severity in patients with chronic hepatitis C

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Feb 7, 2008 (publication date) through Apr 19, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Feb 7, 2008; 14(5): 701-708
Published online Feb 7, 2008. doi: 10.3748/wjg.14.701

Table 1 Primers used for reverse transcription-PCR of the HVR and NS5B genes of HCV

Region/Nucleotide position	Primer set	Forward/Reverse	Sequence (5’-3’)	Amplicon size (bp)
HVR	Outer	Forward	GTGGTTTGGATGTGTGTGGATGAAC
2040-2443		Reverse	TATTGCACGTCCACGATGTTCTG	403
	Inner	Forward	GGCTCCCCCTTGCAACATCT
		Reverse	GTGGAGGTGTATGAGCCCTGTTG
NS5B	Outer	Forward	TGGGGATCCCGTATGATACCCGCTGCTTTGA
8301-8593		Reverse	GGCGGAAT (T/A) CCTGGTCATAGCTCCGTGAA	293
	Inner	Forward	CTCAAC (C/T) GTCACTGAACAGGACAT
		Reverse	CCACGAC (T/C) AGATC (A/G) TCTCCG

Citation: Kumar D, Malik A, Asim M, Chakravarti A, Das RH, Kar P. Influence of quasispecies on virological responses and disease severity in patients with chronic hepatitis C. World J Gastroenterol 2008; 14(5): 701-708
URL: https://www.wjgnet.com/1007-9327/full/v14/i5/701.htm
DOI: https://dx.doi.org/10.3748/wjg.14.701