Role of chemokines and their receptors in viral persistence and liver damage during chronic hepatitis C virus infection

Figure 7 Correlation between liver inflammation and frequency of intrahepatic CCR5 and CXCR3 expressing T cells in chronic hepatitis C. A: Representative photomicrographs of liver immunostaining for CD8 from two chronic hepatitis C patients with different grades of inflammation. CD8 cells are stained in dark by the immunoperoxidase technique. Original magnification 400X. A patient with lobular activity (LA) 1 and porto-periportal activity (PPA) 1 showed little staining while a patient with LA 3 and PPA 3 presented intense CD8 staining; B: FACScan^® dot-plots of intrahepatic lymphocytes from these two patients after staining with CD8-Pe and with either CCR5-Cy5 or CXCR3-FITC mAbs. In the upper right quadrant are represented the double positive cells. The frequency of CCR5/CXCR3 expressing CD8+ cells is higher in the patient with the higher histological liver inflammation.