Acute and persisting Th2-like immune response after fractionated colorectal γ-irradiation

Figure 5 Persistence of Th2 polarization at 6 mo. Expression of chemokine receptors CXCR3 and CCR4 (A) and transcription factors T-bet and Gata3 expression (B) in colon mucosa was assessed 26 wk after the end of cumulative dose of 52Gy. Aspects of immune response that changed long after irradiation included IFNγ and IP-10 expression and downregulation of signal transducer and activator 1 (STAT1) (C). Immunoblot showing STAT1 in total protein extracts from mucosa colon. GAPDH levels are used as internal standards and relative densitometric data are analysed following normalization to the control. Dot plots of four experiments on different samples are shown. Data are the mean ± SE; ^aP < 0.05 vs control.