Acute and persisting Th2-like immune response after fractionated colorectal γ-irradiation

Figure 4 Long-term impairment of inflammatory response. Delayed effects of the radiation schedule on expression of TNFα, MCP-1 and iNOS in colon mucosa 26 wk after the end of cumulative dose of 52 Gy (A). The quantification of target genes was normalized to the reference gene HPRT. Immunostaining for macrophages (B) and apoptotic cells (C) in the colon: Fractionated irradiated group shows no intense ED1-positive macrophages in the lamina propria and no significant modification of the apoptotic cell number 26 wk post-irradiation. Data are mean ± SE, ^aP < 0.01; ^bP < 0.005 vs control.