Potentially probiotic bacteria induce efficient maturation but differential cytokine production in human monocyte-derived dendritic cells

Figure 3 The effect of bacterial dose on the maturation of moDCs. MoDCs were stimulated with bacteria:host cell ratio of 2:1, 10:1, or 40:1. Cells from four different donors were collected, pooled, and stained with antibodies against (A) HLA class II and (B) CD86. The expression of co-stimulatory molecules was analyzed by flow cytometry. The values represent mean fluorescence intensities (MFIs). Results from a representative experiment out of two are shown. Dotted lines indicate respective isotype controls. The staining profiles of HLA class II/CD86 double-positive (percentages included in the profiles) cells (C). GAS: S. pyogenes; THS: S. thermophilus THS; Bb99: B. breve Bb99; ARH74: L. lactis subsp. cremoris ARH74.