Biological impact of hepatitis B virus X-hepatitis C virus core fusion gene on human hepatocytes

Table 1 Sequences of gene primers

Target gene	Primer sequences	Product (bp)
HBV X gene	P1: 5’-ATCTGTCGACATGGCTGCTAGGCTGTGCT G-3’	465
	P2: 5’-CGCGGATATCTTAGGCAGAGGTGAAAAAGT TG-3’
HCV C gene	P3: 5’-ACTGGTCGACATGAGCACGAATCCTAAACCT C-3’	576
	P4: 5’-ACTGGATATCTTAGGCTGAAGCGGGCAC AG-3’
HBV X-Linker fragment	P1: 5’-ATCTGTCGACATGGCTGCTAGGCTGTGCT G-3’	510
	P2’: 5’-GCTGCCGCCACCGCCGCTTCCGCCACCGCCGCTTGCCACCGGCAGAGGTGAAAAAGTTGCA-3’
Linker-HCV C fragment	P3’: 5’-GGTGGCGGTGGAAGCGGCGGTGGCGGCGGAAGCGGCGGTGGCGGCAGCATGAGCACGAATCCTAAACCTC -3’	621
	P4: 5’-ACTGGATATCTTAGGCT GAAGCGGGCAC AG-3’
HBV X-HCV C fusion gene	P1: 5’-ATCTGTCGACATGGCTGCTAGGCTGTGCT G-3’	1086
	P4: 5’-ACTGGATATCTTAGGCTGAAGCGGGCAC AG-3’
c-myc	5’-TTCGGGTAGTGGAAAACCAG-3’	203
	5’-CAGCAGCTCGAATTTCTTCC-3’
β-actin	5’-GTGGGGCGCCCCAGGCACCA-3’	540
	5’CTTCCTTAATGTCACGCACGATTTC-3’