Ellagic acid induces apoptosis through inhibition of nuclear factor κB in pancreatic cancer cells

Figure 4 Ellagic acid does not directly affect the function of isolated mitochondria. Mitochondria were isolated from MIA PaCa-2 cells cultured in the absence of ellagic acid. A: Oxygen consumption was measured using a Clark-type electrode connected to an oxygen meter; B: Mitochondrial membrane potential (ΔΨm) was monitored in the presence of 2 &mgr;mol/L tetraphenyl phosphonium (TPP⁺) using a TPP⁺-sensitive electrode connected to an amplifier. Protonophore CCCP (10 &mgr;mol/L) was added to dissipate ΔΨm; C: Cytochrome C levels were measured in the incubation medium and the mitochondrial pellet by Western blot analysis. The results are representative of 3 independent experiments.