Inhibition of CXCR4 activity with AMD3100 decreases invasion of human colorectal cancer cells in vitro

Figure 2 Effect of AMD3100 on viability of CRC SW480 cells. After 24 h incubation, cells growing in 96-well plates were treated with AMD3100 for 2 h. CXCL12 was added at 20 ng/mL per day, and the MTT assay revealed that in serum-free medium or the absence of CXCL12, AMD3100-induced inhibition was relatively weak. CXCL12-induced cell proliferation was significantly suppressed by 100 and 1000 ng/mL AMD3100 in SW480 cells. Cell viability was not significantly affected by 10 ng/mL AMD3100 (compared to the unstimulated group). Data are mean ± SD of eight wells after 3 d incubation. Bars indicate mean ± SD of triplicate experiments.