Role of soluble factors and three-dimensional culture in in vitro differentiation of intestinal macrophages

Figure 3 Cell ELISA of MO/MAC antigen expression after seven days of co-culture with IEC conditioned media. A: Freshly elutriated MO were incubated in conditioned medium of the IEC line WiDr or control media (left graph) of HT-29 and control media (right graph) for seven days. Expression of the housekeeping antigen β2M was determined an extinction is given as absolute value; B: CD68, CD14 and CD16 antigen expression of MO incubated in conditioned medium of the IEC line WiDr or non-conditioned control medium (left graph) or HT-29 and control medium (right graph) after seven days was also determined by cell ELISA. Values are standardized on β₂M-extinction.