Frequent loss of heterozygosity in two distinct regions, 8p23.1 and 8p22, in hepatocellular carcinoma

Table 1 Informative microsatellite markers were selected and used in this study

No.	Locus	Markers	Genetic map(cM)	Forward	Reverse	PCR productsize (bp)	result1
1	8p23.3	D8S7	Not listed	ACCCTGACAGCAGAGGTTTC	ACCCTGACGTTCTCCCAGTA	250-252	ni
2	8p23.2	D8S1164	Not listed	CACAAATCAGATTTTTGAAGTTGC	GGGTTAGACGGACAACCTCA	225	ni
3	8p23.2	D8S264	0.7	ACATCTGCGTCGTCTTCATA	CCAACACCTGAGTCAGCATA	121-145	in
4	8p23.2	D8S262	4.3	AGCTCAAAAGCGAAGGTGAT	GGCAACAAAGTGAGATCCTG	114-128	in
5	8p23.1	sts-X53793	Not listed	TCGACTACCCAGTGGTCTTG	GTTCAAAATGCTTGCTCGC	127	ni
6	8p23.1	D8S1742	Not listed	CCCCCACCAAGACACA	CTCAAGGGATATGAAGGGCA	130	in
7	8p23.1	D8S277	8.2	GATTTGTCCTCATGCAGTGT	ACATGTTATGTTTGAGAGGTCTG	121	in
8	8p23.1	D8S1918	Not listed	GAATGTCATGCTGGGAACG	GTAGCTCTCAAAGCAAATTATGAGC	108	ni
9	8p23.1	D8S1819	10	TCACTGAGGGACTTGGC	CGTGCTGAGAATGAGACC	207	in
10	8p23.1	D8S1140	Not listed	GACAACATCCGATAATGCTG	GAGGACATCTAGATAATTGGAAGA	378	ni
11	8p23.1	D8S503	16.2	GGTTACGAGTTTTGTCCTTTG	GAAACAAACCAATGTAGGAGTG	136	in
12	8p23.1	D8S1672	Not listed	AACTGAGATCACGCCACTCC	CCCATTGGTTTTAGAGTGGC	149	ni
13	8p23.1	AFM234ve1	Not listed	TACCGCAAAACACACCA	GCAGCCTTAGTTGACAACA	245	ni
14	8p23.1	D8S2045	Not listed	CCGATTGCTTCATCGGGAC	CGCCTCCTCCTCTGAAATCCT	120	ni
15	8p23.1	D8S1130	22.4	GAAGATTTGGCTCTGTTGGA	TGTCTTACTGCTATAGCTTTCATAA	145	in
16	8p23.1	D8S1946	Not listed	GCACAAGATCAGAGAGGTTGTG	GAGGAGAGATGGTGTTGGGA	102	ni
17	8p23.1	D8S1640	Not listed	TGCAGTCTGCGGGAGTTC	AGCAGGGTGACTGTAAAGAAGG	175	ni
18	8p23.1	D8S2060	Not listed	CTCTCCGGGAATGTAATACTGC	GAGCTGGGAGTTACTGCCTG	256	ni
19	8p23.1	D8S552	26.4	CCTGTACCATACCCCTGTATC	AAGGTTTGAATCTCTCAGTGG	132	in
20	8p23.1	D8S1109	26.4	TTCTCAGAATTGCTCATAGTGC	TCAGCTCCTCTTCTGCTGAT	241	in
21	8p23.1	D8S2066	Not listed	TTTTCTCCATCCGGTGACTC	CCAACTACGGCATGGTTTCT	175	ni
22	8p23.1-22	D8S1106	26.5	TTGTTTACCCCTGCATCACT	TTCTCAGAATTGCTCATAGTGC	149	ni
23	8p22	D8S1451	Not listed	AACCTAAGGTTCTGTGCTACATCA	AACTTACCAAGGCCGTTTAGG	149	ni
24	8p22	EST465487	Not listed	TTTGTTTGGGTGGAGGACTC	TGGACATCTGCCTAGGTCCT	250	ni
25	8p22	D8S1647	Not listed	CCAGAATTTTGAAATAGATTCATCC	AAATTTTGTAAATATCAGTGTTCCG	174	ni
26	8p22	stSG29388	32	GCAGTGAGATTTTGCTTCTGG	ATGAACATTCAATGAATCAGCA	125	ni
27	8p22	D8S1713	Not listed	CAGGGGCTGATTGTCAGAAC	GTGGCTGTCACCAAGGTCTC	113	ni
28	8p22	SGC33312	Not listed	AGGGCCTTGGGAACACTC	TCAGTTTAAATGGATGGTTTTTACT	137	ni
29	8p22	D8S2080	Not listed	GACTCAAAGAGAACCTGCCG	TAGGTTGGTGAGCACACGTC	132	ni
30	8p22	D8S2081	Not listed	ACCCAGTTACAGCACTGTAATATCA	CTCTACCCCGAAATGATGGA	147	ni
31	8p22	SHGC-24261	Not listed	AAGCAGAGATAAGCCCGACA	TTTCTTTAGATGGAGTCCATTGC	123	ni
32	8p22	SHGC-52401	Not listed	ACAGGATAGTGTTAGGCTCACATG	CATTCTCTGTATCTTTTGGGGG	120	ni
33	8p22	D8S254	Not listed	TGCCGGACATACATTAGTGA	TTGTAAACACCACAAGCAGG	65-75	in
34	8p22	D8S2001	Not listed	GACATTGAATTCCAGTATTTGTGC	GGACAAATGCCACTGCAAC	138	ni
35	8p22	SHGC-5873	Not listed	GACACACACATACAGAAAACCA	CTTACCATGAATGGAGCTTG	225	ni
36	8p22	D8S261	35.8	TGCCACTGTCTTGAAAATCC	TATGGCCCAGCAATGTGTAT	128	in
37	8p22	AFM234vf4	Not listed	GGGCACAGGCATGTGT	GGCTGCATTCTGAAAGGTTA	260-272	ni
38	8p22	D8S1948	Not listed	TTACAAAACATACCCAGTGTTTGG	CTTTTTAGTGCTTGAGACTGTCTCC	110	ni
39	8p22	D8S2028	Not listed	TCAAAAGTTTTGTTTCTATTCAGGG	TTTTTTCTGTTCCCCTCCG	178	ni
40	8p22	D8S258	40.3	CTGCCAGGAATCAACTGAG	TTGACAGGGACCCACG	144-154	in
41	8p22	D8S1949	Not listed	TGTCTTACAGCTCTCCCTCTCC	CAGTAAGGATCACCAAGACAAGG	106	ni
42	8p22	D8S1983	Not listed	ATTGGAAGAGGCAAATGGTG	TATGTACTGGATGAAGCAGGACA	175	ni
43	8p22	D8S1786	Not listed	CGAAAGATTGAGACCCCAT	GTTTCCACACCGAAGCC	209	ni
44	8p22	D8S298	42.7	AGGCTTACACCCATGGACC	ACGCAGCACACAACATCAT	155-167	in
45	8p21.3	D8S2050	Not listed	TGCCAATATCAGTGGAAGAGG	TCCTTTTTCCCTTGTTGCC	162	ni
46	8p21.3	D8S1752	Not listed	TCCTGGATCAGGCAGAAA	TCAGAGTTGGGTGAGCGA	140	in
47	8p21.3	D8S1734	44.9	GCTATCCACTTGTCCCAGA	AGCCCAGAAATAAACCCTC	114	in
48	8p21.2	D8S2256	Not listed	GTGTCTTGAGATACTGGTGA	GAGAAATGTCTTTGTTGAGG	101	ni
49	8p21.2	D8S2259	Not listed	TGAAAGCCTGTTTAGAGAGA	CTATTGCCCTGTGTTTTGCC	105	ni
50	8p21.2	D8S1220	Not listed	TTCCGTATACACATGCACCC	TAGCAGCCAGACACAGGAGC	90	ni
51	8p21.1	D8S1445	Not listed	GCAACAGAGCGAGACTCCGTC	AAGCTTACATTCTGGGTGAC	117-139	in
52	8p21.1	D8S2261	Not listed	GTATTTATTCCACAAGCATCTTA	CAACCCCATCAGTCTCTCTAAT	204	ni
53	8p21.1	D8S1444	Not listed	TTCTTCTAGATTTCCTACTA	CATTTGTTAAAAGTACAACC	91	x
54	8p21	D8S2249	Not listed	TCCACCCATTTCAGCCTTTC	CTAAAACATTTAACTTTCATT	101	ni
55	8p21	D8S2248	Not listed	ATACAGGTAGGTGAGGGCAA	TTCTGATGCTCTTCTGGAGT	136	ni
56	8p21	D8S2247	Not listed	CATTGTGGTGGAGGTCGGAG	TTCCCCCATCCCATCTGAG	122	ni
57	8p21	D8S2262	Not listed	ATGTTTTGTTCATGGGTCTTT	AAGAAAAAGGGAAGGGGCAGT	98	ni
58	8p21	D8S339	Not listed	TAGATGTTACCATTTCAC	GATTAGATCTTGGATCAG	162	ni
59	8p21	D8S2245	Not listed	CCTCTTTATCCCACTTTTCAG	CATTTTACGAATATAAGCATCC	138	ni
60	8p21	D8S2244	Not listed	ACAACTAAAGGACTTAAAGG	GACAAGAAAAGAACAAATGG	145	ni
61	8p21	D8S2246	Not listed	TAACCTGTGAATGAGAATAC	TGACAGTTTTGAGAGAATCC	169	ni

¹ni: noninformative; in: informative.