Proteasome inhibition-induces endoplasmic reticulum dysfunction and cell death of human cholangiocarcinoma cells

Figure 5 The translational inhibitor cycloheximide inhibits MG132 induced cell death in KMCH cells. The translational inhibitor, cycloheximide (CHM) 50 μmol/L, completely inhibited MG132 1 μmol/L induced apoptosis in KMCH cells co-treated with both agents for 24 h. Cell death was assessed by morphologic criteria using DAPI and fluorescence microscopy. Data represent the mean ± SE of three separate studies.