Proteasome inhibition-induces endoplasmic reticulum dysfunction and cell death of human cholangiocarcinoma cells

Figure 2 Caspase 3/7 activity and apoptosis in MG132 and staurosporine (SP) treated KMCH cells A: Cells were treated with MG132 1 μmol/L and SP 5 μmol/L for 16 h. Caspase 3/7 activity was increased SP cells (P < 0.05). In contrast, caspase activity was not different for MG132 treated cells vs controls. B: Cell death was assessed by morphologic criteria using DAPI and fluorescence microscopy. Cells were treated with SP or MG1321 for 24 h in the absence or presence of the pancaspase inhibitor, QVD at 5 μmol/L. Note QVD blocked staurosporine, but not MG132, induced apoptosis. Data represent the mean ± SE of three separate studies.